īoth types of naturally occurring CKs were discovered, quantified and characterized. CKs can regulate all stages of plant development and directly affect plant growth processes. The presented approach is applicable for the synthesis of any other desired -labelled C6-substituted purine derivatives.Ĭytokinins (CKs) are naturally occurring substances in plants derived from adenine with either an aromatic (ARCK) or isoprenoid (ISCK) side chain at the N 6-position. The chemical identity and purity of prepared compounds were confirmed by physico-chemical analyses (TLC HRMS HPLC–MS 1H, 13C, 15N NMR). Compared to current methods, optimized syntheses of 6-amino-9H-purine (adenine) and 6-chloro-9H-purine (6-chloropurine) were performed to achieve more effective, selective and generally easier approaches. In this work, 11 -labelled C6-purine derivatives were prepared, among them 5 aromatic ( 4, 5, 6, 7, 8) and 3 isoprenoid ( 9, 10, 11) CKs. Therefore, defined standards labelled with stable isotopes are required for precise metabolic profiling and quantification of CKs, as well as in vivo elucidation of CK biosynthesis in various plant species. They are typically found at minute concentrations in plant tissues containing very complicated biological matrices. Cytokinins (CKs) and their metabolites and derivatives are essential for cell division, plant growth regulation and development.
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